Thermo Fisher Scientific CD4 Monoclonal Antibody (RPA-T4), Brilliant Violet 421, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (RPA-T4), Brilliant Violet 421, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test
Publications: -

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 496, 615, 661, 737, 805
• Brilliant Violet 480, 605, 650, 711
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue 510, 585, 690
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

Description:
The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by thymocytes, mature T-helper cells, and at low levels on monocytes. CD4 serves as a receptor for HIV, and RPA-T4 blocks HIV binding and mixed lymphocyte reactions. This antibody recognizes a different epitope than OKT4 and does not cross-block.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and validated for flow cytometry using normal human peripheral blood cells. Recommended usage: 5 µL (0.25 µg)/test in 100 µL final volume, with 10⁵–10⁸ cells/test.

Fluorochrome Information:
Brilliant Violet™ 421 (BV421) emits at 423 nm and is optimized for cytometers with a violet (405 nm) laser. Ensure instrument compatibility.

Staining Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.

Laser Information:
Excitation: 407 nm; Emission: 423 nm; Laser: Violet Laser.

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD4 antigen recognizes MHC class II molecules and acts as a co-receptor for HIV. It is expressed primarily on T-helper lymphocytes, and also on monocytes, macrophages, and dendritic cells. CD4 expression is found in thymus, lymph nodes, tonsils, spleen, and certain non-lymphoid tissues. It plays a key role in T-cell activation and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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