Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Brilliant Violet 421, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Brilliant Violet 421, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
• Publications: View 1 publication

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805
• Brilliant Violet 480–786
• eFluor 450, 506, 615, 660
• FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PerCP-Cyanine5.5
• Super Bright 436–780
• Request custom conjugation

Product Specific Information

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as either a heterodimer (CD8αβ) or homodimer (CD8αα).

• Expression: Thymocytes, mature αβ T cells (CD8αβ), γδ T cells, intestinal IELs, dendritic cells (CD8αα)
• Function: CD8 binds to MHC class I and associates with protein tyrosine kinase p56lck, playing a role in T cell development and activation.

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes, ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Antibody titration recommended for optimal performance.

Fluorophore: Brilliant Violet™ 421 (BV421)

• Excitation: 407 nm
• Emission: 423 nm
• Laser: Violet (405 nm)

Buffer Recommendations:
Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) when combining multiple polymer dye-conjugated antibodies to minimize non-specific interactions.

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company. Powered by Sirigen™.

Target Information

CD8 (Cluster of Differentiation 8)

• Type I transmembrane glycoprotein of the immunoglobulin receptor family
• Functions as a co-receptor for MHC class I molecules
• Expressed on T cells, NK cells, monocytes, and dendritic cells
• CD8α chain essential for MHC-I binding
• Activation leads to cytotoxic T lymphocyte (CTL) formation and pathogen/tumor clearance
• Differentiation enhanced by IL-2, IL-12, TGF-β1

For Research Use Only. Not for diagnostic procedures or resale without authorization.

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