Thermo Fisher Scientific PAX2 Recombinant Rabbit Monoclonal Antibody (JJ082-08)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JJ082-08

Immunogen

Recombinant protein within Human Pax2 aa 161-357 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PAX2,uniProtId:Q02962-1,ncbiNodeId:9606,antigenRange:161-357,antigenLength:417,antigenImageFileName:MA5-32432_PAX2_Q02962-1_Rabbit.svg,antigenImageFileNamePDP:MA5-32432_PAX2_Q02962-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2809710

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

The pax genes are a family of transcription factors that are active in specific tissues during early embryonic development. Pax family members possess a DNA-binding domain encoded by the paired box. Because paired domain containing genes encode transcription factors, they are capable of executing a genetic program. Several pax genes have also been associated with developmental mutations including: pax-3, which is associated with Waardenburg syndrome, pax-6, which is associated with Aniridia, and pax-2, which is associated with Wilms tumor. During embryogenesis, Pax-2 is expressed in the developing kidney. In particular, the pax-2 gene is expressed in condensing metanephric mesenchyme and in early epithelial structures derived from mesenchyme; however, pax-2 mRNA and protein levels are rapidly down regulated as the tubular epithelium matures. Although Pax-2 is down regulated during renal epithelium maturation, Pax-2 expression persists in the undifferentiated epithelium of Wilmstumors. Persistent expression of Pax-2 in Wilms tumors occurs frequently and correlates with the proliferation of poorly differentiated epithelial cells in these tumors. Interestingly, expression of the Wilms` tumor suppresser protein, WT1, coincides with down-regulation of the pax-2 gene; thus, suggesting that WT1 can directly repress pax-2 transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.