Thermo Fisher Scientific CD9 Monoclonal Antibody (eBioKMC8 (KMC8)), APC, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1 µg/test
• Publications: View 16 publications

Product Specifications

Available Formats:

• Unconjugated
• Alexa Fluor 700
• eFluor 450
• FITC
• PE
• Super Bright 600
• Request custom conjugation

Product Specific Information

The eBioKMC8 monoclonal antibody reacts with mouse CD9, a 24 kDa member of the transmembrane 4 superfamily (tetraspanins). CD9 is expressed on multiple cell types including:

• Monocytes, macrophages, platelets
• Early B cells, activated B and T cells
• Dendritic cells, eosinophils, basophils
• Endothelial cells, myoblasts, neuroblasts

On T cells, CD9 functions as a co-stimulatory molecule on naive T cells. CD9-deficiency in oocytes leads to sterility due to defective gamete fusion. In macrophages, CD9 associates with FcγRs to modulate phagocytosis and inflammatory responses. In B cells, CD9 serves as a marker for marginal zone B cells, B1 cells, and plasma cells. In dendritic cells, CD9 facilitates MHC II molecule association. Expression levels may vary by donor.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse bone marrow cells at ≤1 µg/test.

• A test = antibody amount to stain cells in 100 µL final volume
• Cell number: 10⁵–10⁸ cells/test (empirical optimization recommended)
• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD9 antigen is a glycoprotein expressed on developing B lymphocytes, platelets, monocytes, eosinophils, basophils, activated T lymphocytes, neurons, and glial cells.
CD9 belongs to the tetraspanin family, spanning the membrane four times. It regulates cell activation and aggregation in pre-B cells and platelets, possibly via integrin CD41/CD61 (GPIIb/GPIIIa).

Functions and roles:

• Cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, sperm-egg fusion
• Cell attachment, proliferation, and MHC II association in dendritic cells
• Suppression of cancer cell motility and metastasis (acts as a metastasis suppressor)

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

제품 이미지