Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), APC, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), APC, eBioscience™

Applications

• Flow Cytometry (Flow): 5 µL (0.06 µg)/test
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• T-Cell Activation (TCA): –
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Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC-eFluor 780
• Biotin, eFluor 450, FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow Series
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright Series (436–780)
• Request custom conjugation

Product Specific Information

The OKT8 monoclonal antibody reacts with the human CD8a molecule, a 32–34 kDa cell surface receptor expressed on thymocytes and subsets of mature T cells and NK cells. CD8 binds to MHC class I and associates with protein tyrosine kinase p56lck, playing a role in T-cell development and activation.
OKT8 does not compete with clones RPA-T8 and HIT8a for binding, indicating distinct epitopes.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for use at 5 µL (0.06 µg)/test (100 µL final volume; 10⁵–10⁸ cells/test).
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD8 is a type I transmembrane glycoprotein functioning as a co-receptor with TCR for MHC class I molecules. It is expressed as a heterodimer (CD8αβ) or homodimer (CD8αα) on T cells, NK cells, monocytes, and dendritic cells. CD8 engagement triggers Lck recruitment, leading to lymphokine production and cytotoxic T lymphocyte (CTL) activation.
Activated CTLs play a key role in pathogen and tumor cell clearance. Differentiation of naive CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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