Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), APC, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), APC, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC-eFluor 780
• Biotin
• Brilliant UV & Brilliant Violet series
• eFluor 450, 506, 615, 660
• FITC
• PE, PE-Cyanine series, PerCP-eFluor series
• Super Bright series
• NovaFluor Blue, Red, Violet, Yellow series
• Functional Grade
• Request custom conjugation

Product Specific Information

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8αβ or CD8αα dimers. CD8 binds MHC class I and, through association with p56lck, plays a role in T cell development and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometry of mouse thymocyte or splenocyte suspensions (≤0.125 µg/test)
• Recommended Conditions: Final volume 100 µL; cell number 10⁵–10⁸ cells/test
• Excitation/Emission: 633–647 nm / 660 nm (Red Laser)
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD8 is a type I transmembrane glycoprotein of the immunoglobulin family, functioning as a co-receptor with TCR for MHC class I molecules. It is expressed on T cells, NK cells, monocytes, and dendritic cells. CD8 engagement with MHC-I/peptide complexes triggers Lck recruitment, lymphokine production, and cytotoxic T lymphocyte activation, essential for pathogen and tumor clearance.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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