Thermo Fisher Scientific ESAM Monoclonal Antibody (1G8), PE, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 4 publications 4 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

1G8

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_891537

Product Specific Information

Description: The 1G8 monoclonal antibody reacts with mouse ESAM (endothelial cell-selective adhesion molecule). ESAM is a 55-kDa membrane protein composed of 2 extracellular Ig domains, a single transmembrane domain, and a 120 amino acid cytoplasmic domain. ESAM is related to members of the junctional adhesion molecule (JAM) family, and is expressed on endothelial cells and activated platelets. ESAM at endothelial tight junctions plays a role in the regulation of vascular permeability, in response to agents such as VEGF or extravasating neutrophils.

Applications Reported: This 1G8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 1G8 antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ESAM is a 55-kDa membrane protein composed of 2 extracellular Ig domains, a single transmembrane domain, and a 120 amino acid cytoplasmic domain. ESAM is related to members of the junctional adhesion molecule (JAM) family, and is expressed on endothelial cells and activated platelets. ESAM at endothelial tight junctions plays a role in the regulation of vascular permeability, in response to agents such as VEGF or extravasating neutrophils.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.