Thermo Fisher Scientific Embigin Monoclonal Antibody (G7.43.1), PE, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

G7.43.1

Conjugate

PE PE PE

View additional formats

• Unconjugated
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2016637

Product Specific Information

Description: This G7.43.1 monoclonal antibody reacts with mouse embigin (gp70), a single transmembrane glycoprotein that is a member of the Ig superfamily similar to basigin and EMMPRIN. It contains two Ig domains thought to be involved in cellular adhesion and tissue remodeling. Expression of embigin is high before day 10 in fetal development. In the adult it is expressed on myeloid cells and T cells but not B cells. During hematopoeisis, embigin is expressed on HSCs and CLPs (common lymphoid progenitors) but absent on pro- and pre-B cells. In recent studies Pax 5 has been shown to repress embigin expression.

Applications Reported: This G7.43.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This G7.43.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Embigin is a 327 amino acid regulatory protein belonging to the immunoglobulin superfamily class of CAMs, and has two Ig-like (immunoglobulin-like) V- type domains. In situ hybridization analysis shows that Embigin has specific carbohydrate epitopes and plays an important role in early postimplantation embryogenesis. A highly glycosylated transmembrane glycoprotein, Embigin acts as a regulator of cell/ECM interactions during development and homeostasis of normal adult tissues. Transfection studies suggest its role in promotion of integrin-mediated cell-substratum adhesion. Widely expressed in most of the tissues and embryonal carcinoma cells, prostate and mammary cancer cell lines, it may also help in the classification of human carcinomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.