Thermo Fisher Scientific TdT Monoclonal Antibody (19-3), PE, eBioscience

Thermo Fisher Scientific TdT Monoclonal Antibody (19-3), PE, eBioscience

Applications

• Intracellular staining
• Flow Cytometry

Tested Dilution: ≤ 0.5 µg/test
Publications: View 2 publications

Product Specifications

Product Specific Information

Description:
This 19-3 monoclonal antibody recognizes human and mouse terminal-deoxynucleotidyl transferase (TdT), a 60 kDa polymerase responsible for the template-independent addition of N-nucleotides at gene segment junctions of developing lymphocytes. Expression is found in the nucleus of immature lymphocytes but not in mature lymphocytes or non-lymphoid cells. TdT plays a critical role during TCR and Ig gene rearrangement. Regulation of expression in the mouse thymus correlates with T cell selection; decreased or absent after positive selection. Additionally, TdT has been shown to be present in lymphoma or lymphoblastic leukemia cells.

Applications Reported:
This 19-3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested:
This antibody has been tested by intracellular staining using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5521-00) followed by flow cytometric analysis of mouse thymocytes.
Recommended usage: ≤ 0.5 µg/test (100 µL final volume).
Cell number range: 10^5–10^8 cells/test.
Careful titration is recommended for optimal performance.

Excitation / Emission / Laser:

• Excitation: 488–561 nm
• Emission: 578 nm
• Laser: Blue, Green, Yellow-Green

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

Terminal Deoxynucleotidyl Transferase (TdT) is a DNA polymerase located in the cell nucleus which catalyzes polymerization of deoxynucleotides at the 3′ hydroxyl ends of oligo or polydeoxynucleotide initiators and functions without a template. TdT is considered a highly specific marker for the diagnosis and classification of acute lymphoblastic lymphoma/leukemias. Determination of TdT expression is valuable for differentiating lymphoblastic lymphoma and Burkitt’s lymphoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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