Thermo Fisher Scientific TdT Monoclonal Antibody (19-3), PE, eBioscience
TdT 단백질을 인식하는 Mouse monoclonal antibody (Clone 19-3)로, PE 형광표지되어 있습니다. 인간 및 마우스 시료에 반응하며 Flow Cytometry에 적합합니다. PBS buffer에 보관하며 4°C, 암조건에서 저장합니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific TdT Monoclonal Antibody (19-3), PE, eBioscience
Applications
- Intracellular staining
- Flow Cytometry
Tested Dilution: ≤ 0.5 µg/test
Publications: View 2 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 19-3 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1963620 |
Product Specific Information
Description:
This 19-3 monoclonal antibody recognizes human and mouse terminal-deoxynucleotidyl transferase (TdT), a 60 kDa polymerase responsible for the template-independent addition of N-nucleotides at gene segment junctions of developing lymphocytes. Expression is found in the nucleus of immature lymphocytes but not in mature lymphocytes or non-lymphoid cells. TdT plays a critical role during TCR and Ig gene rearrangement. Regulation of expression in the mouse thymus correlates with T cell selection; decreased or absent after positive selection. Additionally, TdT has been shown to be present in lymphoma or lymphoblastic leukemia cells.
Applications Reported:
This 19-3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested:
This antibody has been tested by intracellular staining using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5521-00) followed by flow cytometric analysis of mouse thymocytes.
Recommended usage: ≤ 0.5 µg/test (100 µL final volume).
Cell number range: 10^5–10^8 cells/test.
Careful titration is recommended for optimal performance.
Excitation / Emission / Laser:
- Excitation: 488–561 nm
- Emission: 578 nm
- Laser: Blue, Green, Yellow-Green
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
Terminal Deoxynucleotidyl Transferase (TdT) is a DNA polymerase located in the cell nucleus which catalyzes polymerization of deoxynucleotides at the 3′ hydroxyl ends of oligo or polydeoxynucleotide initiators and functions without a template. TdT is considered a highly specific marker for the diagnosis and classification of acute lymphoblastic lymphoma/leukemias. Determination of TdT expression is valuable for differentiating lymphoblastic lymphoma and Burkitt’s lymphoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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