Thermo Fisher Scientific OCT3/4 Monoclonal Antibody (EM92), PE, eBioscience
Oct3/4 단백질을 인식하는 EM92 클론의 Rat 모노클론 항체로, PE 형광 표지된 제품입니다. 인간 및 마우스 시료에서 줄기세포 관련 연구에 활용됩니다. Flow Cytometry용으로 검증되었으며, 세포 내 염색에 적합합니다. 4°C 암조건 보관, 동결 금지.
- 카탈로그번호
- 12-5841-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific OCT3/4 Monoclonal Antibody (EM92), PE, eBioscience
Thermo Fisher Scientific OCT3/4 Monoclonal Antibody (EM92), PE, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.5 µg/test
View 5 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | EM92 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_914364 |
Additional Formats
Product Specific Information
The EM92 monoclonal antibody reacts with mouse and human Oct3/4, encoded by the Pou5F1 gene. Oct3/4 is a POU domain-containing transcription factor critical for maintaining embryonic stem (ES) and induced pluripotent stem (iPS) cells in a pluripotent state. It is expressed by ES cells, embryonic germ cells, and embryonic carcinoma cell lines.
Reduction of Oct3/4 expression in inner cell mass leads to dedifferentiation to trophoectoderm, while increased expression induces differentiation to mesoderm and primitive endoderm. Oct3/4 regulates genes such as FGF-4, UTF1, Sox2, Fbx15, Rex1, and osteopontin, and frequently acts synergistically with Sox2.
Oct3/4 expression can be negatively regulated by retinoic acid treatment or removal of leukemia-inhibitory factor (LIF).
- Applications Reported: Intracellular staining followed by flow cytometric analysis
- Applications Tested: Intracellular staining and flow cytometric analysis of F9 embryonal carcinoma cells using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00)
Refer to BestProtocols® for staining protocol (Protocol B: One-step protocol for intracellular (nuclear) proteins).
Recommended usage: ≤0.5 µg per test (100 µL final volume). Cell number may vary from 10⁵–10⁸ cells/test. Antibody titration is advised for optimal performance.
Excitation: 488–561 nm
Emission: 578 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
POU5F1 (Oct-4) is a maternally expressed octamer-binding transcription factor crucial in early developmental stages. It plays a key role in maintaining pluripotency and has applications in stem cell generation and regenerative medicine.
Artificial stem cells (iPS cells) can be created by expressing POU5F1 along with Sox2, Klf4, and c-Myc in fibroblasts. Recent studies show iPS cells can be generated via plasmid expression without viral introduction, improving safety for therapeutic use.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.