Thermo Fisher Scientific CD369 (Clec7a, Dectin-1) Monoclonal Antibody (bg1fpj), PE, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

bg1fpj

Immunogen

Baculovirus-expressed Fc-containing protein

Conjugate

PE PE PE

View additional formats

• APC
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572632

Product Specific Information

Description: The monoclonal antibody bg1fpj recognizes mouse CD369, also known as Dectin-1 or CLEC7A, a type II transmembrane protein in the C-type lectin family. The ligand is beta1, 3-glycan. Beta glycans are a main component in polysaccharides and specifically beta1,3-linked carbohydrates are found in the cell wall of fungi, plants and some bacteria. CD369 has been found to bind Candida, Aspergillis, Pneumocystis, Coccidioides and Mycobacterium. Calcium dependent binding to ligand signals through syk via the ITAM domain in the cytoplasmic portion and results in endocyotosis/phagocytosis and production of cytokines (IL-2, IL-6, IL-10, TNF alpha, IL-1 beta, IL-23). A direct role in Th17 and Th1 differentiation has been attributed to CD369 signaling. CD369 is expressed predominantly on myeloid cells (neutrophils, monocytes, macrophages, and dendritic cells). Low levels have also been reported on lymphocytes and mucosal epithelium.

Applications Reported: This bg1fpj antibody has been reported for use in flow cytometric analysis.

Applications Tested: This bg1fpj antibody has been tested by flow cytometric staining of mouse lysed whole blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CLEC7A, also known as dectin-1, is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily and is predominantly expressed on myeloid cells. It is a small glycoprotein type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif (ITAM). CLEC7A functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Upon fungal exposure, CLEC7A activates Syk tyrosine kinase, triggering a massive oxidative burst through the formation of reactive oxygen species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.