Thermo Fisher Scientific TRIM25 Polyclonal Antibody
TRIM25 단백질을 표적하는 Rabbit Polyclonal Antibody로 Western Blot 및 Immunocytochemistry에 적합. Human 시료 반응성, 항원 친화 크로마토그래피로 정제. 액상 형태, 1 mg/mL 농도, PBS 버퍼 및 sodium azide 포함. 연구용으로만 사용 가능.
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Thermo Fisher Scientific TRIM25 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 15 amino acid peptide from near the amino terminus of human TRIM25 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11155571 |
Product Specific Information
- Suggested positive control: Human thymus tissue lysate
- PA5-20411 can be used with blocking peptide PEP-0528
Target Information
TRIM25, also known as estrogen responsive finger protein (EFP), is a member of the RING finger-B box-coiled coil family and a downstream target of estrogen receptor α.
Mice lacking this gene show underdeveloped uterus and reduced estrogen responsiveness, indicating its essential role in estrogen-dependent proliferation.
TRIM25 expression increases in breast carcinoma and promotes tumor growth through its E3 ubiquitin ligase activity targeting the negative cell cycle checkpoint protein 14-3-3σ.
Recent studies show that TRIM25 E3 ubiquitin ligase is crucial for the RIG-1 signaling pathway in antiviral innate immunity.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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