Thermo Fisher Scientific TRIM25 Polyclonal Antibody
TRIM25 단백질을 검출하기 위한 Rabbit Polyclonal 항체. Western blot과 Immunocytochemistry에 사용 가능. Human 및 Mouse 반응성. 항원 친화 크로마토그래피로 정제된 액상 형태. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | 20 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 19 amino acid peptide from near the carboxy terminus of human TRIM25 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long term storage store at -20°C. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11157247 |
Product Specific Information
- A suggested positive control is HeLa cell lysate.
- PA5-20410 can be used with blocking peptide PEP-0527.
Target Information
TRIM25, also known as estrogen responsive finger protein (EFP), is a member of the RING finger-B box-coiled coil family and a downstream target of estrogen receptor α.
Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness, demonstrating that this protein is essential for estrogen-dependent proliferation.
TRIM25 expression is increased in breast carcinoma, promoting tumor growth by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3σ.
Recent studies indicate that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway, contributing to host antiviral innate immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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