Thermo Fisher Scientific E2F1 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,500–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–5 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Based on 100% sequence identity, this antibody is predicted to react with Mouse

Target Information

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and the action of tumor suppressor proteins, and is also a target of the transforming proteins of small DNA tumor viruses.
E2F proteins contain conserved domains including:

• DNA binding domain
• Dimerization domain (interacts with DP proteins)
• Transactivation domain enriched in acidic amino acids
• Tumor suppressor protein association domain (within the transactivation domain)

E2F1, along with E2F2 and E2F3, has an additional cyclin binding domain. E2F1 binds preferentially to retinoblastoma protein (pRB) in a cell-cycle dependent manner and can mediate both cell proliferation and p53-dependent or independent apoptosis.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.