Thermo Fisher Scientific Phospho-KAP-1 (Ser824) Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.

Target Information

The KRAB (Kruppel-associated box) domain is approximately 45 amino acids long and functions as a transcriptional repression domain found in many transcription factors. Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes have been identified in the human genome.
These proteins repress transcription through interactions with KAP-1 (KRAB-associated protein 1), an 835 amino acid polypeptide containing a RING finger, B boxes, and a PHD finger. KAP-1 forms complexes with KRAB-domain transcription factors to enhance repression efficiency.
KAP-1 interacts directly with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1), targeting KRAZ1 to centromeric heterochromatin foci containing HP1α, thereby regulating transcriptional repression.
Mutant KAP-1 proteins that bind KRAB but not HP1 result in random KRAZ1 distribution and strong transcriptional activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.