Thermo Fisher Scientific Phospho-KAP-1 (Ser824) Polyclonal Antibody
Phospho-KAP-1 (Ser824) 폴리클로날 항체로 인간 KRAB-Associated Protein 1의 인산화된 Ser824 부위를 인식합니다. Western blot, IHC, ICC/IF, IP 등 다양한 응용에 사용 가능하며, 액상 형태로 4°C에서 보관합니다. 연구용으로만 사용됩니다.
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- A300767Ax (2개 옵션)
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphorylated peptide representing a portion of Human KRAB-Associated Protein 1 surrounding Serine 824 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
The KRAB (Kruppel-associated box) domain is approximately 45 amino acids long and functions as a transcriptional repression domain found in many transcription factors. Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes have been identified in the human genome.
These proteins repress transcription through interactions with KAP-1 (KRAB-associated protein 1), an 835 amino acid polypeptide containing a RING finger, B boxes, and a PHD finger. KAP-1 forms complexes with KRAB-domain transcription factors to enhance repression efficiency.
KAP-1 interacts directly with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1), targeting KRAZ1 to centromeric heterochromatin foci containing HP1α, thereby regulating transcriptional repression.
Mutant KAP-1 proteins that bind KRAB but not HP1 result in random KRAZ1 distribution and strong transcriptional activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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