Thermo Fisher Scientific CD3 Monoclonal Antibody (17A2), FITC, eBioscience
마우스 CD3 복합체를 인식하는 17A2 단클론 항체로, FITC 형광 표지되어 있습니다. Flow cytometry 및 IHC(F) 분석에 적합하며, T 세포 활성화 연구에 활용됩니다. 488 nm 흥분, 520 nm 방출 특성을 가지며, 4°C 암소 보관이 권장됩니다.
- 카탈로그번호
- 11-0032-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD3 Monoclonal Antibody (17A2), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 |
| Immunohistochemistry (IHC) | - | 13 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 |
| Immunohistochemistry (Frozen) (IHC (F)) | 10 µg/mL | 2 |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | 5 |
| Flow Cytometry (Flow) | 0.25 µg/test | 125 |
| T-Cell Activation (TCA) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Bacteria, Fungi, Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 17A2 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572430 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395
- Brilliant UV 496
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 650
- eFluor 450
- eFluor 506
- eFluor 660
- Functional Grade
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600
- Super Bright 702
- Super Bright 780
- Request custom conjugation available
Product Specific Information
Description:
The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits gamma, delta, and epsilon are essential for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates intracellular signaling leading to cellular activation and proliferation.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes at ≤0.25 µg/test.
Tested by immunohistochemistry on frozen mouse spleen at ≤10 µg/mL.
Optimal antibody titration is recommended for each assay.
Optical Properties:
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
The CD3 subunit complex is crucial for transducing antigen-recognition signals into T cells and regulating TCR surface expression.
It includes CD3 gamma, delta, epsilon, and zeta subunits—members of the immunoglobulin superfamily encoded on chromosome 11.
CD3 plays roles in TCR-induced growth arrest, survival, and proliferation.
CD3 antigen is expressed on 68–82% of normal peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells in the cerebellum, but not on B or NK cells.
Decreased T lymphocytes may be observed in autoimmune diseases.
Defects in CD3 genes are associated with CD3 immunodeficiency.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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