Thermo Fisher Scientific CD3 Monoclonal Antibody (HIT3a), FITC, eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

Description:
The HIT3a monoclonal antibody reacts with human CD3ε, a 20 kDa subunit of the TCR complex. Along with other CD3 subunits (γ and δ), the ε chain is required for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR with HIT3a initiates intracellular signaling leading to T-cell activation and proliferation.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells.
Recommended usage: 5 µL (1 µg) per test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test.

Fluorescence Properties:
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 subunit complex is crucial for transducing antigen-recognition signals into T cells and regulating TCR surface expression. CD3 subunits (γ, δ, ε, ζ) are members of the immunoglobulin superfamily encoded on human chromosome 11.
They associate with intracellular signaling molecules via tyrosine-based motifs, mediating T-cell activation, proliferation, and survival.
CD3 is expressed on 68–82% of normal peripheral blood lymphocytes and 65–85% of thymocytes but absent on B and NK cells.
Defects in CD3 genes are associated with CD3 immunodeficiency.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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