Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), FITC, eBioscience
인간 CD3 복합체의 epsilon 서브유닛을 인식하는 OKT3 단클론 항체로, FITC 형광 표지를 통해 T세포 분석에 적합합니다. Flow cytometry에 최적화되어 있으며, 5 µL/test로 사용됩니다. PBS 기반 완충액에 BSA와 sodium azide가 포함되어 있습니다.
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- 11-0037-4x (2개 옵션)
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- pk
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Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 3 |
| Immunohistochemistry (IHC) | - | 2 |
| Immunocytochemistry (ICC/IF) | - | 4 |
| Flow Cytometry (Flow) | 5 µL (1 µg)/test | 45 |
| Functional Assay | - | 1 |
| Immunomicroscopy (IM) | - | 1 |
| T-Cell Activation (TCA) | - | 5 |
| Miscellaneous PubMed | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Rhesus monkey |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT3 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2016601 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 563, 615, 661, 737, 805
- Brilliant Violet 421, 605
- eFluor 450, 506, 660
- Functional Grade
- NovaFluor Blue 510, 585
- NovaFluor Yellow 590, 660, 730
- PE, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available upon request
Product Specific Information
The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. It exhibits potent immunosuppressive properties in vivo and has been proven effective in treating renal, heart, and liver allograft rejection.
CD3 subunits (gamma, delta, epsilon) are required for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells. Crosslinking of TCR initiates intracellular signaling leading to activation and proliferation. Clones OKT3 and SK7 recognize different epitopes.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells
Recommended Usage: 5 µL (1 µg) per test (100 µL final volume)
Excitation: 488 nm; Emission: 520 nm; Laser: Blue
Filtration: 0.2 µm post-manufacturing filtered
Target Information
The CD3 complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. CD3 subunits (gamma, delta, epsilon, zeta) are structurally related members of the immunoglobulin superfamily encoded on chromosome 11.
CD3 is expressed on 68–82% of normal peripheral blood lymphocytes and 65–85% of thymocytes, but not on B or NK cells. Defects in CD3 genes are associated with CD3 immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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