Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), FITC, eBioscience

Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), FITC, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV / Violet / Yellow series
• eFluor 450, 506
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain, along with other CD3 subunits (gamma and delta), is essential for proper assembly and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells. Crosslinking of TCR via immobilized UCHT1 triggers intracellular signaling leading to activation and proliferation.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on normal human peripheral blood cells at ≤1 µg/test (100 µL final volume). Optimal titration recommended.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

The CD3 complex is crucial for transducing antigen-recognition signals in T cells and regulating TCR surface expression. It includes CD3 gamma, delta, epsilon, and zeta subunits, encoded by genes on chromosome 11. These subunits mediate signal transduction via tyrosine-based motifs. CD3 is expressed on most peripheral blood lymphocytes and thymocytes but not on B or NK cells. Defects in CD3 genes are linked to immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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