Thermo Fisher Scientific Human HGF Recombinant Protein
인간 유래 HGF 재조합 단백질로 세포 성장, 이동, 형태형성 연구에 적합합니다. 곤충 세포에서 발현되며 순도 98% 이상입니다. 기능적 활성 검증 완료, ED50 1–20 ng/mL. PBS에 동결건조 형태로 제공되며 -20°C 보관 권장.
- 카탈로그번호
- PHG0xxx (3개 옵션)
- 카테고리
- Protein
- 판매단위
- pk
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Applications
Western Blot (WB)
- Tested Dilution: Assay-dependent
Immunohistochemistry (IHC)
- Tested Dilution: Assay-dependent
ELISA
- Tested Dilution: Assay-dependent
Functional Assay
- Tested Dilution: Assay-dependent
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Control (Ctrl)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Published species | Human |
| Expression System | Insect cells |
| Amino acid sequence | Human HGF recombinant protein is a heterodimer consisting of a 463 amino acid alpha chain and a 234 amino acid beta chain |
| Molecular weight | 70 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >98% by SDS-PAGE |
| Endotoxin concentration | <0.1 ng/µg |
| Activity | ED50 = 1 - 20 ng/mL; determined by the dose-dependent proliferation of monkey 4MBr-5 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
Carrier-Free
- Source: (BTI-Tn-5B1-4) Hi-5 Insect cells
- Reconstitution:
Centrifuge the vial briefly before opening to bring the contents to the bottom.
Reconstitute the lyophilized protein in sterile PBS containing 0.1% human serum albumin.
Make further dilutions in low endotoxin medium or buffered solution containing a carrier protein such as heat-inactivated FCS or tissue culture grade BSA. - Storage:
Store the lyophilized protein at ≤ -20°C, preferably desiccated.
Upon reconstitution, apportion into working aliquots and store at ≤ -20°C.
Avoid repeated freeze-thaw cycles.
Target Information
Hepatocyte growth factor (HGF) regulates cell growth, motility, and morphogenesis by activating a tyrosine kinase signaling cascade through binding to the c-Met receptor. It is secreted by mesenchymal cells and acts as a multifunctional cytokine on epithelial cells. HGF plays a central role in angiogenesis, tumorigenesis, and tissue regeneration. It is secreted as an inactive polypeptide that is cleaved into a 69-kDa alpha-chain and 34-kDa beta-chain, forming an active heterodimer via a disulfide bond. This protein belongs to the plasminogen subfamily of S1 peptidases but lacks protease activity. Multiple transcript variants encoding different isoforms are produced by alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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