Thermo Fisher Scientific Human FGF-18 Recombinant Protein
Human FGF-18 재조합 단백질로 세포 성장 및 분화 연구에 활용 가능. E. coli 발현 시스템에서 정제되어 >95% 순도 확보. Balb/c 3T3 세포 증식으로 활성 검증됨. Lyophilized 형태로 안정적 보관 및 재구성 용이. 연구용으로만 사용 가능.
- 카탈로그번호
- PHG0234
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | Human FGF-18 recombinant protein contains 173 amino acids |
| Molecular Weight | 21.2 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% by SDS-PAGE |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 ≤ 10 ng/mL; determined by the dose-dependent proliferation of Balb/c 3T3 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | 10 mM Tris, pH 8 |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
- Reconstitution: Briefly centrifuge vial before opening. Reconstitute in 5 mM Tris, pH 8.0, to 0.1–1.0 mg/mL. Further dilutions should be made in aqueous buffers with 0.1–1.0% BSA.
- Storage: Lyophilized preparation stable at 2°C–8°C; store at -20°C for long term (preferably desiccated). After reconstitution, stable up to one week at 2°C–8°C. For maximal stability, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Target Information
FGF18 is a member of the fibroblast growth factor (FGF) family, which possesses broad mitogenic and cell survival activities. These proteins are involved in embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion.
In vitro, FGF18 induces neurite outgrowth in PC12 cells. Studies in mouse and chick show it acts as a pleiotropic growth factor stimulating proliferation in tissues such as liver and small intestine. Knockout studies in mice indicate its role in regulating proliferation and differentiation of midline cerebellar structures.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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