Thermo Fisher Scientific AP2M1 Polyclonal Antibody
AP2M1 단백질을 인식하는 Thermo Fisher Scientific의 Chicken Polyclonal 항체입니다. Western blot, IHC, ICC/IF에 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. 인간, 마우스, 랫트 반응성이 있으며, 연구용으로 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | 2.5 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 20 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Chicken / IgY |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 16 amino acid peptide near the carboxy terminus of human AP2M1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154294 |
Product Specific Information
- A suggested positive control is human kidney tissue lysate.
- PA5-20745 can be used with blocking peptide PEP-0859.
Target Information
The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family.
The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, responsible for proton pumping during endosome and lysosome acidification.
AP2M1 associates with the HIV-1 protein Nef, suggesting that Nef may utilize the AP-2 complex to enhance endocytosis of CD4 and class I MHC.
AP2M1 may also regulate intracellular trafficking and the function of CTLA-4 protein.
At least two isoforms of AP2M1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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