Thermo Fisher Scientific SH3BP4 Polyclonal Antibody
SH3BP4 단백질을 인식하는 Thermo Fisher의 토끼 폴리클로날 항체로, WB 및 IHC에 적합합니다. 인간, 마우스, 랫트 반응성이 있으며 항원 친화 크로마토그래피로 정제되었습니다. 냉장 보관 가능하며 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (IHC) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | An 18 amino acid peptide from near the carboxy terminus of human SH3BP4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long term storage store at -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153405 |
Product Specific Information
- A suggested positive control is rat lung tissue lysate.
- PA5-20747 can be used with blocking peptide PEP-0861.
Target Information
SH3BP4 was initially isolated as a novel cDNA using differential display analysis of cultured human corneal fibroblasts. The protein contains three Asn-Pro-Phe (NPF) motifs, an SH3 domain, a PXXP motif, a bipartite nuclear targeting signal, and a tyrosine phosphorylation site.
SH3BP4 interacts with endocytic proteins such as clathrin, dynamin, and the transferrin receptor (TfR), localizing to TfR-containing coated pits and vesicles. It is involved in cargo-specific control of clathrin-mediated endocytosis, particularly the internalization of TfR. At least two isoforms of SH3BP4 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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