Thermo Fisher Scientific Goat anti-Rat IgG (H+L) Secondary Antibody, FITC
염소 유래의 항 Rat IgG (H+L) 2차 항체로 FITC 형광 표지되어 있습니다. ICC/IF 및 유세포분석에 적합하며, 높은 특이성과 민감도를 제공합니다. 친화 크로마토그래피로 정제되었으며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 2.5 µg/mL |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.8, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535643 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats Available
- AP
- Biotin
- HRP
- NovaFluor Blue 510, 555, 585, 610-70S, 660-120S
- NovaFluor Yellow 590, 610, 660
- PE
- Rhodamine
- TRITC
- WesternDot 625
- Custom conjugation available upon request
Product Specific Information
Each lot of antibody is tested for sensitivity using ELISA and for specificity by immunoelectrophoresis (IEP).
Target Information
Anti-Rat secondary antibodies are affinity-purified and exhibit high specificity for rat immunoglobulins. These antibodies are valuable for detection, sorting, or purification of target molecules. Secondary antibodies enhance assay versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to one primary antibody. Typically, they are produced by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to ensure high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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