Thermo Fisher Scientific GST Polyclonal Antibody, Biotin

Thermo Fisher Scientific GST Polyclonal Antibody, Biotin

Applications and Tested Dilution

• Western Blot (WB): 1:500–1:2,500
• Immunohistochemistry (IHC): 1:1,000–1:5,000
• ELISA: 1:10,000–1:50,000
• Immunoprecipitation (IP): 1:2,000–1:10,000

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below.
• Avoid repeated freeze/thaw cycles.
• Centrifuge if solution is not clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.
• Immunoelectrophoresis assay shows a single precipitin arc against anti-biotin, anti-Goat Serum, and purified Glutathione-S-Transferase (Schistosoma japonicum).
• Cross-reactivity with GST from other sources may occur but has not been specifically determined.

Target Information

GST (Glutathione S-transferase) is a 26 kDa protein found in both eukaryotes and prokaryotes, catalyzing various reactions.
The GST gene fusion system utilizes GST to create fusion proteins that can be purified via their high affinity for glutathione.
Fusion proteins are valuable tools for studying direct protein–protein interactions.
The GST gene fusion system has been introduced into numerous expression vectors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.