Thermo Fisher Scientific GFP Polyclonal Antibody, Biotin
GFP 단백질을 인식하는 Thermo Fisher Scientific의 Biotin 결합 폴리클로날 항체로, WB, IHC, ELISA, IP에 적합합니다. 고순도 친화 크로마토그래피 정제 제품으로 높은 특이성과 재현성을 제공합니다. 연구용으로 GFP 융합 단백질 검출에 이상적입니다.
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- 600-106-215
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- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,500 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:50,000–1:80,000 |
| Immunoprecipitation (IP) | 1:2,000–1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below. Avoid freeze/thaw cycles.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only immediately before use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-biotin, and purified or partially purified Green Fluorescent Protein (Aequorea victoria) Serum.
No reaction was observed against Human, Mouse, and Rat Serum Proteins.
Target Information
The jellyfish Aequorea victoria contains Green Fluorescent Protein (GFP Tag) that emits light in its bioluminescence reaction. GFP is widely used as a reporter protein for gene expression in both eukaryotic and prokaryotic organisms and as a protein tag in cell culture and multicellular organisms.
As a fusion tag, GFP enables localization studies, movement tracking, and analysis of subcellular compartment dynamics. This technology has provided significant insights into the physiological activities of living cells.
GFP is a 27 kDa monomeric protein that forms a fluorescent pigment autocatalytically. The wild-type protein absorbs blue light (max 395 nm) and emits green light (peak 508 nm) without requiring additional proteins or cofactors. GFP fluorescence is stable, species-independent, and suitable for multiple applications including gene expression monitoring, protein localization, yeast two-hybrid interaction studies, and FRET experiments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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