Thermo Fisher Scientific GFP Polyclonal Antibody, AP

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Store vial at 4°C before opening. Do not freeze.
• Stable at 4°C as an undiluted liquid; dilute only prior to immediate use.
• Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase, and purified and partially purified GFP (Aequorea victoria) serum.
• No reaction was observed against Human, Mouse, and Rat serum proteins.

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in its bioluminescence reaction. GFP is widely used as a reporter protein for gene expression in both eukaryotic and prokaryotic systems, and as a protein tag in cell culture and multicellular organisms.
As a fusion tag, GFP enables localization studies, tracking of protein movement, and analysis of subcellular compartment dynamics. GFP technology has advanced understanding of physiological activities in living cells.

• GFP is a 27 kDa monomeric protein forming a fluorescent pigment autocatalytically.
• Absorbs blue light (max 395 nm) and emits green light (peak 508 nm).
• Fluorescence is stable, species-independent, and suitable for various applications.
• Commonly used for monitoring gene expression, protein localization, and in FRET experiments to assess protein-protein interactions.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.