Thermo Fisher Scientific Biotin Polyclonal Antibody, PE
Biotin에 특이적인 염소 폴리클로날 항체로 PE 형광단백질로 표지됨. Western blot, IHC, ELISA, IP 등 다양한 응용에 사용 가능. 고순도 친화 크로마토그래피 정제. 4°C 단기 보관, -20°C 장기 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:20,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | 1:50–1:200 |
| Immunoprecipitation (IP) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH) |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- Restore with deionized water (or equivalent).
- Stable at 4°C as an undiluted liquid; dilute only before immediate use.
- Centrifuge if not clear after standing at room temperature.
- Do not freeze after reconstitution.
- Store reagent in the dark; handle under subdued lighting.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Phycoerythrin, Biotin conjugated IgG, and Biotin conjugated Bovine Serum Albumin.
Target Information
Biotin (Vitamin B7)는 수용성 비타민 B 복합체로, 모든 생물체에서 필수적이며 주로 세균, 효모, 곰팡이, 조류 및 일부 식물에 의해 합성됩니다. Biotin은 유기 화합물 내 탄소 고정 과정에 필요하며, 분자량은 약 244 Da입니다.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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