Thermo Fisher Scientific p38 (MAPK14) Monoclonal Antibody (OTI5A6)

Applications

• Western Blot (WB): 1:500 dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

MAPK14 (p38 alpha) is a member of the stress-activated protein kinase (SAPK) class of MAPKs. The p38 MAPK cascade regulates cellular responses to stress, inflammation, and other stimuli.
Inactive in the nonphosphorylated form, p38 MAPK becomes activated through dual phosphorylation at the Thr-Gly-Tyr motif.
There are four isoforms of p38 MAPK, each differing in tissue expression and affinity for activators and effectors.
Upon exposure to TNF, IL-1, heat shock, or other stimuli, MAPK kinase-3/6 phosphorylates p38 MAPK at Thr180 and Tyr182, leading to activation of transcription factors such as ATF-2, CHOP-1, and MEF-2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.