Thermo Fisher Scientific p38 (MAPK14) Monoclonal Antibody (OTI7H6)

Applications

Western Blot (WB)

• Tested Dilution: 1:500

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

MAPK14 (p38 alpha) is a member of the stress-activated protein kinase (SAPK) class of MAPKs.
The p38 MAPK cascade regulates cellular responses to stress, inflammation, and various stimuli.
p38 MAPK is inactive in its nonphosphorylated form and becomes rapidly activated by dual phosphorylation of the Thr-Gly-Tyr motif.
There are four isoforms of p38 MAPK differing in tissue expression and affinity for upstream activators and downstream effectors.
Upon exposure to TNF, IL-1, heat shock, or other stimuli, MAPK kinase-3/6 phosphorylates Thr180 and Tyr182 in p38 MAPK, leading to activation of transcription factors such as ATF-2, CHOP-1, and MEF-2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.