Thermo Fisher Scientific Amphiregulin Polyclonal Antibody, Biotin, PeproTech
Human Amphiregulin을 인식하는 Biotin 결합 Rabbit Polyclonal Antibody로 ELISA 및 Western Blot에 최적화됨. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 감도를 제공. 연구용으로 EGFR 관련 신호전달 및 암 연구에 활용 가능.
- 카탈로그번호
- 500-P322BT-xxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Amphiregulin Polyclonal Antibody, Biotin, PeproTech
Thermo Fisher Scientific Amphiregulin Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human Amphiregulin |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930407 |
Product Specific Information
AA Sequence of recombinant protein:
SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Amphiregulin. Anti-Human Amphiregulin-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect Human Amphiregulin by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Amphiregulin (500-P322) as a capture antibody, allows detection of at least 2000–4000 pg/mL of Recombinant Human Amphiregulin.
Western Blot:
To detect Human Amphiregulin by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible development reagents, the detection limit for Recombinant Human Amphiregulin is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Package Information:
500-P322BT-1MG will be provided as 2 × 500 µg.
Target Information
Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form is an 84-amino acid glycosylated polypeptide growth regulator generated by proteolytic processing of a 252-amino acid transmembrane precursor.
Seven distinct EGFR ligands include EGF, TGFα, AR, HB-EGF, cripto-1, epiregulin, and betacellulin, all containing a characteristic EGF-like domain with six cysteine residues forming three disulfide-bonded loops.
AR protein is localized in the epithelium of colon, stomach, pancreas, breast, and placenta, and is reportedly overexpressed in cancers of breast, colon, stomach, and pancreas.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
제품 이미지
(이미지 파일명: 500-P322BT-25UG_Amphiregulin_P15514-1_Rabbit.svg)
(이미지 파일명: 500-P322BT-25UG_Amphiregulin_P15514-1_Rabbit_PDP.jpeg)
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