Thermo Fisher Scientific beta-5 Defensin Polyclonal Antibody, Biotin, PeproTech
Human beta-5 Defensin을 인식하는 Biotin 결합 Rabbit Polyclonal Antibody로, Western Blot 및 ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 고순도 재조합 단백질로 면역화된 항체입니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 500-P323BT-xxxx (2개 옵션)
- 판매단위
- pk
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Thermo Fisher Scientific beta-5 Defensin Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human BD-5 |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930413 |
Product Specific Information
AA Sequence of recombinant protein:
GLDFSQPFPS GEFAVCESCK LGRGKCRKEC LENEKPDGNC RLNFLCCRQR I
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human BD-5. Anti-Human BD-5-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect hBD-5 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BD-5 (500-P323) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hBD-5.
Western Blot:
To detect hBD-5 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hBD-5 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Package:
500-P323BT-1MG will be provided as 2 × 500 µg.
Target Information
Defensins (alpha and beta) are cationic peptides with broad-spectrum antimicrobial activity and form an essential part of the innate immune system.
Alpha-defensins differ from beta-defensins by the pairing of their three disulfide bonds. Six human beta-defensins have been identified: BD-1, BD-2, BD-3, BD-4, BD-5, and BD-6.
Beta-defensins are expressed on certain leukocytes and epithelial surfaces. Besides antimicrobial activity, they act as chemoattractants for immature dendritic cells and memory T cells.
These proteins are expressed as C-terminal portions of precursors and released via proteolytic cleavage. Beta-defensins contain a six-cysteine motif forming three intra-molecular disulfide bonds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일: 500-P323BT-25UG_beta-5_Defensin_Q8NG35-1_Rabbit.svg, 500-P323BT-25UG_beta-5_Defensin_Q8NG35-1_Rabbit_PDP.jpeg)
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