Thermo Fisher Scientific Amphiregulin Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific Amphiregulin Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Amphiregulin. Anti-Human Amphiregulin-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human Amphiregulin by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Amphiregulin (500-P322) as a capture antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human Amphiregulin.

Western Blot:
To detect Human Amphiregulin by Western blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible development reagents, the detection limit for Recombinant Human Amphiregulin is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package Information:
500-P322BT-1MG will be provided as 2 × 500 µg.

Target Information

Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84-amino acid glycosylated polypeptide growth regulator, generated by proteolytic processing of a 252-amino acid transmembrane precursor.
Seven different polypeptide ligands derived from distinct genes bind to the extracellular domain of EGFR, including EGF, TGFα, AR, HB-EGF, cripto-1, epiregulin, and betacellulin.
All these growth factors contain a characteristic EGF-like domain defined by six evenly spaced cysteine residues forming three loops via disulfide bonds.
AR protein is localized in epithelium of colon, stomach, pancreas, breast, and placenta, and is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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