Thermo Fisher Scientific beta-5 Defensin Polyclonal Antibody, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.5–2.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
GLDFSQPFPS GEFAVCESCK LGRGKCRKEC LENEKPDGNC RLNFLCCRQR I

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human BD-5. Anti-Human BD-5-specific antibody was purified by affinity chromatography employing an immobilized Human BD-5 matrix.

Sandwich ELISA:
To detect Human BD-5 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human BD-5 (500-P323Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human BD-5.

Western Blot:
To detect hBD-5 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hBD-5 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that form an important part of the innate immune system. The alpha-defensins differ from beta-defensins by the pairing of their three disulfide bonds. Six human beta-defensins have been identified: BD-1, BD-2, BD-3, BD-4, BD-5, and BD-6. Beta-defensins are expressed on certain leukocytes and epithelial surfaces. In addition to antimicrobial activity, they act as chemoattractants for immature dendritic cells and memory T cells. These proteins are expressed as the C-terminal portion of precursors and released via proteolytic cleavage. Beta-defensins contain a six-cysteine motif forming three intra-molecular disulfide bonds.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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