Thermo Fisher Scientific Human IL-4 Recombinant Protein

Applications

• Western Blot (WB): Assay-dependent
• ELISA: Assay-dependent
• Immunoprecipitation (IP): Assay-dependent
• Control (Ctrl): Assay-dependent
• Miscellaneous PubMed (Misc): Refer to publication data

Product Specifications

Product Specific Information

Mature human IL-4 was expressed in Pichia pastoris (strain X-33).
GenBank Accession # P05112, amino acids 25–153.
Protein verification was performed by N-terminal sequencing; the first 10 amino acids are HKCDITLQEI.
Purity is >95% by SDS-PAGE analyses.

Activity: Biological activity determined by proliferation of human TF-1 cells.
Use: For in vitro applications. Perform dose-response assay to determine optimal concentration for specific experiments.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells and serves as a ligand for the interleukin 4 receptor. IL-4 exhibits a broad range of actions and shares overlapping functions with IL-13 due to receptor cross-binding. STAT6 plays a key role in mediating IL-4 immune regulatory signaling.

The IL-4 gene, along with IL-3, IL-5, IL-13, and CSF2, forms a cytokine gene cluster on chromosome 5q. IL-4 is located near IL-13 and IL-5, coordinated by long-range regulatory elements spanning approximately 120 kilobases. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.