Thermo Fisher Scientific Human IL-4 Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species

Human

Published species

Not Applicable

Expression System

P. pastoris

Amino acid sequence

Human IL-4, amino acids 25-153 (Accession # P05112)

Molecular weight

15 kDa

Class

Recombinant

Type

Protein

Purity

>95% by SDS-PAGE

Activity

Determined by the proliferation of human TF-1 cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

purified

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Mature human IL-4 was expressed in Pichia pastoris, X-33. GenBank Accession # P05112, amino acids 25 - 153. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: HKCDITLQEI. Purity is >95% by SDS-PAGE analyses.

Activity: The biological activity of recombinant human IL-4 was determined by measuring the proliferation of the factor-dependent human T-helper cell line, TF-1.

Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.