Thermo Fisher Scientific Human IL-1 beta Recombinant Protein

Applications

• Western Blot (WB): Assay-dependent
• ELISA (ELISA): Assay-dependent
• Immunoprecipitation (IP): Assay-dependent
• Control (Ctrl): Assay-dependent
• Miscellaneous PubMed (Misc): 5 publications available

Product Specifications

Product Specific Information

Mature human IL-1β was expressed in Pichia pastoris (strain KM71H).
GenBank Accession # P01584, amino acids 117–269.
The expressed protein was N-terminal sequenced and begins with APVRSLNCTL.
Purity is >95% by SDS-PAGE analyses.

Activity: Biological activity typically <83.3 pg/mL in proliferation assays of mouse D10S cells.
Use: For in vitro applications only. Perform dose-response assays to determine optimal concentration.
Reconstitution and Dilution:

• Store lyophilized material at 4°C.
• Reconstitute in 0.5 mL distilled or deionized water.
• Dilute in a buffer containing carrier protein, aliquot, and store at or below -70°C.
• Avoid multiple freeze-thaw cycles.
• Suitable buffers: PBS with 1% BSA or neutral media containing <10% FCS.

Target Information

Interleukin-1 beta (IL-1β) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells.
It is synthesized as a 31 kDa inactive pro-form that accumulates in the cytosol.
Cleavage into the active 17 kDa form requires inflammasome activation and caspase-1 processing.
IL-1β signals through IL-1RI and IL-1RII receptors, shared with IL-1α, and its activity can be inhibited by IL-1RA.
It plays a key role in innate host defense, triggering cytokine production and acute-phase responses.
Elevated IL-1β levels are associated with chronic inflammatory conditions, and IL-1β neutralizing antibodies have potential therapeutic value.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.