Thermo Fisher Scientific ID3 Monoclonal Antibody (OTI8B3), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

Members of the Id family of basic helix-loop-helix (bHLH) proteins include ID1, ID2, ID3, and ID4. These proteins are ubiquitously expressed and form heterodimers with class A and B HLH proteins. Due to the absence of the basic region, the heterodimers cannot bind DNA, thereby negatively regulating DNA binding of bHLH proteins.
ID-type proteins inhibit muscle-specific gene expression by blocking E12 and Myo D DNA binding. During muscle differentiation, ID protein levels decrease, enabling E12/E47 to activate myogenic differentiation. Expression of ID proteins is growth factor-dependent, and reduction of ID mRNA via antisense oligonucleotides delays cell cycle reentry after growth factor stimulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.