Thermo Fisher Scientific CD80 (B7-1) Chimeric Recombinant Rabbit Monoclonal Antibody (16C10)
인간 CD80(B7-1)을 특이적으로 인식하는 키메라 재조합 토끼 단클론 항체. 다양한 면역분석(ICC/IF, Flow, ELISA 등)에 사용 가능. B7.1:CD28 경로 억제 기능 보유. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Immunocytochemistry (ICC/IF) | 10 µg/mL | |
| Flow Cytometry (Flow) | 1:100 | |
| ELISA | Assay-dependent | |
| Functional Assay | Assay-dependent | |
| Inhibition Assays | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG, lambda |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 16C10 |
| Immunogen | This antibody was generated by immunization of cynomolgus monkeys with human B7.1 antigen. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074763 |
Product Specific Information
This antibody binds human CD80 and inhibits the B7.1:CD28 pathway.
Target Information
CD80 (B7-1) and CD86 (B7-2) are ligands of T cell costimulatory molecule CD28 and inhibitory receptor CTLA-4 (CD152). Both are expressed on professional antigen-presenting cells and are essential for T cell activation. CD80 is rapidly induced on the surface of activated B cells, EBV-transformed B cell lines, Burkitt’s lymphoma cell lines, follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes by binding to CD28 and CTLA4. Dysregulation of CD80 is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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