Thermo Fisher Scientific CD133 (Prominin-1) Monoclonal Antibody (TMP4), PerCP-eFluor 710, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: 5 µL (0.25 µg)/test
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Product Specifications

Additional Formats

• Alexa Fluor 488
• APC
• PE
• PE-eFluor 610
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The TMP4 monoclonal antibody reacts with human CD133 (Prominin-1), a 120 kDa member of the pentaspan family of proteins that includes Prominin-2. CD133 is expressed in plasma membrane protrusions such as epithelial microvilli and exists in multiple alternatively spliced isoforms with several N-linked glycosylation sites.

Originally identified as an epitope on CD34+ hematopoietic progenitors, CD133 serves as a marker for stem cells and cancer stem cells, including those from colon, brain, and prostate cancers.

The TMP4 antibody binding does not interfere with EMK08 clone binding, indicating recognition of distinct epitopes.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometry on normal human peripheral blood cells. Use 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Fluorochrome Details:
PerCP-eFluor® 710 emits at 710 nm and is excited by the blue laser (488 nm). It can replace PerCP-Cyanine5.5. Recommended filter: 710/50 bandpass (695/40 acceptable). Ensure instrument compatibility.

Light Sensitivity: Protect from light to prevent photo-induced oxidation.

Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark. Clone-specific performance should be verified empirically.

Excitation: 488 nm
Emission: 710 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD133 (AC133) is a 97 kDa glycoprotein with five transmembrane domains, appearing as a 120 kDa band due to glycosylation. It is expressed on immature hematopoietic stem cells but not mature blood cells, and also found in neural and embryonic stem cells.

CD133 expression has been observed in cancers such as leukemia and brain tumors, and at low levels in kidney, pancreas, placenta, and fetal liver. Mutations are linked to retinitis pigmentosa and Stargardt disease. Expression is regulated by at least five tissue-dependent promoters, producing multiple isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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