Thermo Fisher Scientific UBE2G2 Monoclonal Antibody (OTI1B10), TrueMAB
UBE2G2 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC/IF에 적합합니다. 고순도의 친화 크로마토그래피 정제 제품이며, 동결건조 형태로 제공됩니다. 연구용으로 단백질 유비퀴틴화 연구에 활용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B10 |
| Immunogen | Full length human recombinant protein of human UBE2G2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional round of desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The modification of proteins with ubiquitin is a key mechanism for targeting abnormal or short-lived proteins for degradation.
Ubiquitination involves three enzyme classes:
- Ubiquitin-activating enzymes (E1s)
- Ubiquitin-conjugating enzymes (E2s)
- Ubiquitin-protein ligases (E3s)
The UBE2G2 gene encodes a member of the E2 ubiquitin-conjugating enzyme family and shares 100% sequence identity with its mouse counterpart. It is ubiquitously expressed, with high levels in adult muscle. Three alternatively spliced transcript variants encoding distinct isoforms have been identified.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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