Thermo Fisher Scientific CaV1.2 Monoclonal Antibody (S57), PerCP
CaV1.2 단백질을 특이적으로 인식하는 단클론 항체로, PerCP로 표지되어 형광 분석에 적합합니다. Western blot, IHC, ICC, IP 등 다양한 응용에 사용 가능하며, 포유류 시료에 반응합니다. 단백질 G로 정제된 고품질 항체로 연구용으로 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | 1:200 |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Hamster, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S57 |
| Immunogen | Fusion protein amino acids 1507–1733 (intracellular carboxyl terminus) of rabbit Cav1.2 |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 2 mM EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931843 |
Available Formats
Product Specific Information
1 µg/mL of MA5-45389 was sufficient for detection of Cav1.2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 240 kDa (varies with cell background due to glycosylation).
Target Information
CACNA1C encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel complex includes alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Multiple isoforms exist due to alternative splicing or different genes. The protein encoded by CACNA1C binds to and is inhibited by dihydropyridine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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