Thermo Fisher Scientific Phospho-PKC gamma (Thr514) Polyclonal Antibody
PKC gamma 단백질의 Thr514 인산화 부위를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC, ELISA 등 다양한 응용 가능. 뇌 및 척수 신경세포 연구에 적합하며, 신호전달 및 신경퇴행성 질환 연구용으로 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human PKC gamma around the phosphorylation site of Thr514 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The PKC family of serine/threonine kinases, including PRKCG (PKC gamma), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified, differing in structure, tissue distribution, localization, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family, and their activities are Ca²⁺- and phospholipid-dependent.
Protein kinase C can be activated by calcium and diacylglycerol, and PKC family members phosphorylate various protein targets involved in diverse signaling pathways. PKC also serves as a major receptor for phorbol esters, a class of tumor promoters. PKC gamma is expressed exclusively in the brain and spinal cord, localized to neurons, and plays roles in neuronal functions such as long-term potentiation (LTP) and long-term depression (LTD). Knockout studies suggest involvement in neuropathic pain development. Defects in PKC gamma are associated with spinocerebellar ataxia-14 (SCA14).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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