Thermo Fisher Scientific Phospho-eNOS (Thr495) Polyclonal Antibody
Phospho-eNOS (Thr495) 항체로 인간, 마우스, 랫트 시료에 반응합니다. Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용에 적합합니다. Rabbit polyclonal 형식이며 Protein A로 정제되었습니다. -20°C에서 보관하며 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human eNOS around the phosphorylation site of Thr495 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission, and cytotoxicity can all be potentiated through cellular responses to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family, which catalyze the oxidation of L-arginine to produce L-citrulline and NO.
Three NOS isoforms have been cloned:
- Brain or neuronal NOS (b/nNOS, type I)
- Endothelial cell NOS (eNOS, type III)
- Inducible NOS (iNOS, type II)
All NOS isoforms contain calmodulin, NADPH, FAD, and FMN binding domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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