Thermo Fisher Scientific Malate Dehydrogenase Polyclonal Antibody
돼지 심장에서 유래한 Malate Dehydrogenase를 인식하는 Sheep Polyclonal Antibody. Western blot, ELISA, IP에 적합하며, 고순도의 IgG로 정제됨. 4°C 단기 보관, -20°C 장기 보관 권장. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:5,000–1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Pig |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Malate Dehydrogenase [Pig Heart] |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Anti-Malate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography, followed by extensive dialysis against the specified buffer.
Immunoelectrophoresis shows a single precipitin arc against anti-Sheep Serum and purified Malate Dehydrogenase [Pig Heart].
Cross-reactivity with other tissues and species may occur but has not been specifically determined.
Target Information
MDH1 is localized to the cytoplasm and plays a pivotal role in the malate-aspartate shuttle, which coordinates metabolism between cytosol and mitochondria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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