Thermo Fisher Scientific Aspartate Transaminase Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

ELISA (ELISA)

1:5,000-1:25,000

-

Immunoprecipitation (IP)

1:500-1:3,000

-

Product Specifications

Species Reactivity

Pig

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-Aspartate Transaminase antibody was produced by repeated immunizations with Aspartate Transaminase from pig heart. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GOT1,uniProtId:P00503-1,ncbiNodeId:9821,antigenRange:1-413,antigenLength:413,antigenImageFileName:200-601-144-0100_GOT1_P00503-1_Sheep.svg,antigenImageFileNamePDP:200-601-144-0100_GOT1_P00503-1_Sheep_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Aspartate Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Aspartate Transaminase (AST) [Pig Heart]. Cross reactivity against Aspartate Transaminase (AST) from other tissues and species may occur but have not been specifically determined.

Target Information

GOT1 is a glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.