Thermo Fisher Scientific Ras Monoclonal Antibody (4E5)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

2-10 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4E5

Immunogen

A full length of human Ras recombinant protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Pan Ras,uniProtId:P01111-1,ncbiNodeId:9606,antigenRange:1-189,antigenLength:189,antigenImageFileName:603-090_Pan_Ras_P01111-1_House_mouse.svg,antigenImageFileNamePDP:603-090_Pan_Ras_P01111-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:Pan Ras,uniProtId:P01112-1,ncbiNodeId:9606,antigenRange:1-189,antigenLength:189,antigenImageFileName:603-090_Pan_Ras_P01112-1_House_mouse.svg,antigenImageFileNamePDP:603-090_Pan_Ras_P01112-1_House_mouse_PDP.jpeg,sortOrder:2},{targetFamily:Pan Ras,uniProtId:P01116-1,ncbiNodeId:9606,antigenRange:1-189,antigenLength:189,antigenImageFileName:603-090_Pan_Ras_P01116-1_House_mouse.svg,antigenImageFileNamePDP:603-090_Pan_Ras_P01116-1_House_mouse_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS with proprietary stabilizer

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Positive control: Ras full length protein.

Cellular location: Cell membrane, Golgi apparatus membrane.

Target Information

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.