Thermo Fisher Scientific c-Myc Monoclonal Antibody (9B7)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Positive control: Colon adenocarcinoma
• Cellular location: Nucleus

Target Information

The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is frequently activated in various tumor cells and plays a critical role in cellular proliferation, differentiation, apoptosis, and cell cycle progression.

Phosphorylation of c-Myc at Thr58/Ser62 by kinases such as glycogen synthase kinase 3, cyclin-dependent kinase, ERK2, and JNK is associated with cell proliferation and regulation. c-Myc is essential for vasculogenesis and angiogenesis during tumor development.

The c-myc oncogene (p62 c-myc) regulates normal cellular proliferation and differentiation, while deregulated expression induces apoptosis. Antibodies against c-Myc epitopes recognize overexpressed proteins containing Myc epitope tags fused to either amino- or carboxy-termini of target proteins.

c-Myc-, N-Myc-, and L-Myc-encoded proteins are involved in proliferation, differentiation, and neoplastic disease. Amplification of the c-Myc gene has been found in lung, breast, and colon carcinomas, while N-Myc amplification is common in neuroblastomas. Translocation of the c-myc locus on chromosome 8 to immunoglobulin loci on chromosomes 14, 2, or 22 is characteristic of Burkitt’s lymphoma and other B-cell lymphoproliferative conditions. Aberrant expression of c-myc occurs in tumors of various origins, including colorectal, gastric, hepatic, mammary, ovarian, pulmonary, thyroidal, and hematopoietic neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.