
Thermo Fisher Scientific TRMT5 Monoclonal Antibody (OTI1B5), TrueMAB
TRMT5 단백질을 인식하는 Mouse monoclonal antibody. Western blot 및 IHC(P) 검증 완료. 동결건조 형태로 제공되며 재구성 후 약 1 mg/mL 농도. 연구용으로 tRNA 메틸화 효소 TRMT5 탐지에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B5 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–303 of human TRMT5 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
tRNAs contain up to 13–14 nucleotides that are posttranscriptionally modified by highly specific enzymes.
TRMT5 methylates the N1 position of guanosine-37 in selected tRNAs using S-adenosyl methionine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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