Thermo Fisher Scientific TRMT5 Monoclonal Antibody (OTI1B5), TrueMAB

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

OTI1B5

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-303 of human TRMT5 produced in E.coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TRMT5,uniProtId:Q32P41-1,ncbiNodeId:9606,antigenRange:1-303,antigenLength:509,antigenImageFileName:CF809321_TRMT5_Q32P41-1_House_mouse.svg,antigenImageFileNamePDP:CF809321_TRMT5_Q32P41-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 in selected tRNAs using S-adenosyl methionine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.