Thermo Fisher Scientific Goat anti-Human IgG Fc Highly Cross-Adsorbed Secondary Antibody, FITC
Human IgG Fc 인식용 염소 유래 FITC 결합 2차 항체로, 높은 교차흡착 처리로 비특이적 결합을 최소화합니다. ICC/IF 및 유세포분석(Flow Cytometry)에 적합하며, 높은 민감도와 신호 증폭 효과를 제공합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:250 - 1:20,000 |
| Flow Cytometry (Flow) | 1:500 - 1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobin Fc region |
| Conjugate | FITC (Fluorescein Isothiocyanate) |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.8, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535607 |
| Target | IgG Fc |
| Cross Adsorption | Against bovine, horse, mouse and rabbit serum proteins, as well as human IgA and IgM |
| Antibody Form | Whole Antibody |
Available Conjugate Formats
- AP
- Biotin
- HRP
- TRITC
- Custom conjugation available upon request
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of their specified targets.
Secondary antibodies provide versatility for multiple detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, or label conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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