
Thermo Fisher Scientific Goat anti-Human IgG Fc Highly Cross-Adsorbed Secondary Antibody, TRITC
인간 IgG Fc에 특이적인 염소 유래 다클론성 2차 항체로 TRITC 형광 표지. 높은 교차흡착 처리로 비특이적 반응 최소화. 면역세포염색(ICC/IF)에 사용 가능. 친화 크로마토그래피 정제 및 안정적 장기 보관 가능.
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Applications
- Immunocytochemistry (ICC/IF): 1:500–1:20,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulin Fc region |
| Conjugate | TRITC |
| Excitation / Emission Max | 552 / 578 nm |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535611 |
| Target | IgG Fc |
| Cross Adsorption | Against bovine, horse, mouse, and rabbit serum proteins, as well as human IgA and IgM |
| Antibody Form | Whole Antibody |
Additional Formats
- AP
- Biotin
- FITC
- HRP
- Custom conjugation available upon request
Product Specific Information
Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.)
Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Shelf life: 1 year from date of receipt.
Prepare working dilution prior to use and then discard.
Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin human serum proteins, light chains on all human immunoglobulins, human IgA or IgM, or serum proteins from bovine, mouse, or rabbit.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting, or purification of their specified targets.
Secondary antibodies provide increased versatility for various detection systems (e.g., HRP, AP, fluorescence) and greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Most secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species, followed by purification or modification (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to ensure high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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